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ۼ : 15-09-07 10:43
[15.7] ⹮ - High Affinity Host−Guest FRET Pair for Single-Vesicle Content-Mixing Assay: Observation of Flickering Fusion Events
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ȸ : 1,503  

High Affinity HostGuest FRET Pair for Single-Vesicle Content-Mixing Assay: Observation of Flickering Fusion Events

B. Gong, B. Choi, J. Kim, D. Shetty, Y. H. Ko, N. Selvapalam, N. K. Lee, and K. Kim

SNARE Vesicle Fusion: Fluorescence-based single-vesicle fusion assays provide a powerful method for studying mechanisms underlying complex biological processes of SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor)-mediated vesicle fusion and neurotransmitter release. A crucial element of these assays is the ability of the fluorescent probe(s) to reliably detect key intermediate events of fusion pore opening and content release/mixing. Here, we report a new, reliable, and efficient single-vesicle content-mixing assay using a high affinity, fluorophore tagged hostguest pair, cucurbit[7]uril-Cy3 and adamantane-Cy5 as a fluorescence resonance energy transfer (FRET) pair. The power of these probes is demonstrated by the first successful observation of flickering dynamics of the fusion pore by in vitro assay using neuronal SNARE-reconstituted vesicles.

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Total 73
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57 [15.11] ⹮ - Hydrolytic Transformation of Microporous Metal–Organic Frameworks to Hierarchical Micro- and Mesoporous MOFs 11-11 1293
56 [15.8] ⹮ - Porphyrin Boxes: Rationally Designed Porous Organic Cages 09-07 1309
55 [15.7] ⹮ - High Affinity Host−Guest FRET Pair for Single-Vesicle Content-Mixing Assay: Observation of Flickering Fusion Events 09-07 1504
54 [15.4] ⹮ - Self-assembly of Nanostructured Materials through Irreversible Covalent Bond Formation 09-07 1469
53 [15.07] ڹ - High-Conductivity Two-Dimensional Polyaniline Nanosheets Developed on Ice Surfaces 06-29 1499
52 [15.06]ö -Germanium Silicon Alloy Anode Material Capable of Tunable Overpotential by Nanoscale Si Segregation 06-29 1220
51 [15.06]ڹ - Synthesis of Three-Dimensionally Interconnected Sulfur-Rich Polymers for Cathode Materials of High-Rate Lithium-Sulfur Batteries 06-15 1412
50 [15.05]ȱѱ -Two-Photon Absorbing Dyes with Minimal Autofluorescence in Tissue Imaging 06-04 1178
49 [15.05]ȱѱ -A Structural Remedy toward Bright Dipolar Fluorophores in Aqueous Media 05-27 1780
48 [15.04] -N-Heterocyclic Carbene Nitric Oxide Radicals 05-06 1514
47 [15.04]ؿ -Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation 04-23 1507
46 [15.02]⹮ -Reversible Morphological Transformation between Polymer Nanocapsules and Thin Films through Dynamic Covalent Self-Assembly 02-09 1291
45 [14.09]ؿ -Reading Single DNA with DNA Polymerase Followed by Atomic Force Microscopy 09-18 1611
44 [14.08]ö _Crystallization-Induced Properties from Morphology-Controlled Organic Crystals 08-21 1553
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