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ۼ : 15-04-23 17:17
[15.04]ؿ -Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation
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Cytosolic targeting factor AKR2A captures chloroplast outer membrane-localized client proteins at the ribosome during translation

Dae Heon Kim, Jae-Eun Lee, Zheng-Yi Xu, Kyoung Rok Geem, Yun Kwon, Joon Won Park* & Inhwan Hwang*

In eukaryotic cells, organellar proteome biogenesis is pivotal for cellular function. Chloroplasts contain a complex proteome, the biogenesis of which includes post-translational import of nuclear-encoded proteins. However, the mechanisms determining when and how nascent chloroplast-targeted proteins are sorted in the cytosol are unknown. Here, we establish the timing and mode of interaction between ankyrin repeat-containing protein 2 (AKR2A), the cytosolic targeting factor of chloroplast outer membrane (COM) proteins, and its interacting partners during translation at the single-molecule level. The targeting signal of a nascent AKR2A client protein residing in the ribosomal exit tunnel induces AKR2A binding to ribosomal RPL23A. Subsequently, RPL23A-bound AKR2A binds to the targeting signal when it becomes exposed from ribosomes. Failure of AKR2A binding to RPL23A in planta severely disrupts protein targeting to the COM; thus, AKR2A-mediated targeting of COM proteins is coupled to their translation, which in turn is crucial for biogenesis of the entire chloroplast proteome.

ֱ ü ܸ ܹ ϴ ٽ ϴ ܹ AKR2A ʷ ԸǾ, AKR2A ü ܸ ܹ OEP7 ü Ͽ ü ʼ Ѵٴ Ȯ ־. ü ۿ ü ܹ ν ̵ ؼ ˷ ʾҴ. ؿ ռǾ ü ܸ ܹ ν ̰(AFM) ̿ ȣ ۿ Ͽ. ýۿ Ư Ȯϰ, ̸ OEP7 ribosome-nascent chain complex ȣۿ Ͽ. ̸ ؼ ȵ ο Ŀ ü ܸ ܹ OEP7 ν translation ȴٰ Ͽ, co-translation recognition̶ ְڴ. ó Ŀ´̼ǽ(Nature Communications) 4 16 ¶ Ǿ.

 
 

Total 73
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