װ ȭа ΰ

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player

 
ۼ : 13-11-25 22:01
[11.1]⹮ -Supramolecular fishing for plasma membrane proteins using an ultrastable synthetic host–guest binding pair
 ۾ :
ȸ : 1,181  

Supramolecular fishing for plasma membrane proteins using an ultrastable synthetic host–guest binding pair
- D. W. Lee, K. M. Park, M. Banerjee, S. H. Ha, T. Lee, K. Suh, S. Paul, H. Jung, J. Kim, N. Selvapalam, S. H. Ryu and K. Kim

Membrane proteomics, the large-scale global analysis of membrane proteins, is often constrained by the efficiency of separating and extracting membrane proteins. Recent approaches involve conjugating membrane proteins with the small molecule biotin and using the receptor streptavidin to extract the labelled proteins. Despite the many advantages of this method, several shortcomings remain, including potential contamination by endogenously biotinylated molecules and interference by streptavidin during analytical stages. Here, we report a supramolecular fishing method for membrane proteins using the synthetic receptor–ligand pair cucurbit[7]uril–1-trimethylammoniomethylferrocene (CB[7]–AFc). CB[7]-conjugated beads selectively capture AFc-labelled proteins from heterogeneous protein mixtures, and AFc-labelling of cells results in the efficient capture of membrane proteins by these beads. The captured proteins can be recovered easily at room temperature by treatment with a strong competitor such as 1,1-bis(trimethylammoniomethyl)ferrocene. This synthetic but biocompatible host–guest system may be a useful alternative to streptavidin–biotin for membrane proteomics as well as other biological and biotechnological applications.

ܹ и ̿Ǵ ƺ-̿ƾ ս ΰս Ŀ μ üϴ ʷ Ͽ. ̿Ͽ μ ǥ ܹ Ŀ ũ 带 ̿Ͽ и ȸ ־, Ͽ ܹ и ȿ Ӹ ƴ϶ ʴ ܹ ɼ ߴ ߰Ͽ. ý ̿ϸ ܹ м ɷ ų ϸ, ̴ , ۿ Ư ۿϴ ž ϴ ũ ⿩ ϰ ִ.


 
 

Total 78
ȣ     ۾ ¥ ȸ
33 [13.09]ö -Copper-Vapor-Assisted Chemical Vapor Deposition for High-Quality and Metal-Free Single-Layer Graphene 09-11 1353
32 [13.09] -Poly(norepinephrine): Ultrasmooth Material-Independent Surface Chemistry and Nanodepot for Nitric Oxide 09-04 1390
31 [13.07]ڹ -Fast Low Voltage Electroactive Actuators Using Nanostructured Polymer Electrolytes 07-30 1823
30 [13.07] -Photothermally Triggered Cytosolic Drug Delivery via Endosome Disruption Using a Functionalized Reduced Graphene Oxide 07-17 1602
29 [13.07]ڹ -Blue-Emitting Self-Assembled Polymer Electrolytes for Fast, Sensitive, Label-Free Detection of Cu(II) Ions in Aqueous Media 07-08 1278
28 [13.06]ö -Catalyst-free Direct Growth of a Single to a Few Layers of Graphene on a Germanium Nanowire for the Anode Material of a Lithium Battery 06-19 1470
27 [13.4]⹮ -Free-Standing, Single-Monomer-Thick Two-Dimensional Polymers through Covalent Self-Assembly in Solution 11-25 1340
26 [13.3]⹮ -Supramolecular Velcro for Reversible Underwater Adhesion 11-25 1193
25 [13.01] -Active Role of Proton in Excited State Intramolecular Proton Transfer Reaction 06-19 1533
24 [13.01]μ -Reversible and Cyclical Transformations between Solid and Hollow Nanostructures in Confined Reactions of Manganese Oxide and Silica within Nanosized Spheres 02-06 1755
23 [12.07] -Bioreducible polymers for gene silencing and delivery 09-06 1880
22 [12.07]ؿ - Following the DNA Ligation of a Single Duplex Using Atomic Force Microscopy 08-01 1700
21 [12.06]μ - Electroless Pt Deposition on Mn3O4 Nanoparticles via the Galvanic Replacement Process 07-03 2004
20 [12.06]Ž± -Surface-Dependent, Ligand-Mediated Photochemical Etching of CdSe Nanoplatelets 06-14 1706
19 [12.05]⹮ -In Situ Supramolecular Assembly and ModularModification of Hyaluronic Acid Hydrogels for 3D Cellular Engineering 11-25 1056
 1  2  3  4  5  6  

Content on this page requires a newer version of Adobe Flash Player.

Get Adobe Flash player